ARS | Publication request: GROWTH OF AND ENZYME PRODUCTION BY TRICHODERMA REESEI ON CORN FIBER FRACTIONS

Submitted to: Biotechnology for Fuels and Chemicals Symposium Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: May 12, 2004
Publication Date: May 12, 2004
Citation: Li, X., Dien, B.S., Cotta, M.A. 2004. Growth of and enzyme production by Trichoderma reesei on corn fiber fractions [abstract]. Biotechnology For Fuels And Chemicals. p. 25.

Technical Abstract: Corn fiber is the fibrous by-product of wet-mill corn processing that has promise as a lignocellulosic biomass feedstock for fuels and other bio-products. It consists typically of about 20% starch, 14% cellulose, and 30% hemicellulose in the form of arabinoxylan. We have fractionated crude corn fiber (CCF) into destarched corn fiber (DSCF), corn fiber cellulose (CFC), and corn fiber arabinoxylan (CFAX). Shake-flask cultivation has demonstrated that Trichoderma reesei QM9414 and Rut C-30 are able to grow on the CCF, DSCF, CFC, or CFAX and secrete a number of hydrolytic enzymes. Enzyme assays and SDS-PAGE analyses showed that enzyme levels varied significantly between the two strains of T. reesei and among the four substrates. Data on the saccharification of corn fiber components by various enzyme preparations will also be presented.