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United States Department of Agriculture

Agricultural Research Service

Title: Lactic Acid Bacteria Culture with Wide in Vitro Antibacterial Spectrum by Bacteriocin-Like Activity and Probiotic Effect in Vivo

item Tellez, G - UNIV OF ARKANSAS
item Vincente, J - UNIV OF ARKANSAS
item Blore, P - UNIV OF ARKANSAS
item Donoghue, Ann
item Donoghue, Dan - UNIV OF ARKANSAS
item Hargis, B - UNIV OF ARKANSAS

Submitted to: Food Safety Consortium Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: September 30, 2003
Publication Date: October 2, 2003
Citation: Nava, G.M., Tellez, G.I., Vincente, J.L., Blore, P., Donoghue, A.M., Donoghue, D.J., Hargis, B.M. 2003. Lactic acid bacteria culture with wide in vitro antibacterial spectrum by bacteriocin-like activity and probiotic effect in vivo. [abstract]. Food Safety Consortium Proceedings. 2003 CDROM.

Technical Abstract: Mechanisms by which probiotic supplements affect the microecology of the intestinal tract are not well understood. In the intestinal-ecological concept, bacteriocins seem to be a powerful tool that can inhibit pathogenic bacteria. In the present investigation, several in vitro and in vivo studies were performed to demonstrate the antagonistic effect of a novel candidate lactic acid bacteria (LAB) probiotic culture (PHL-1) with wide in vitro antibacterial spectrum by bacteriocin-like activity. The 2 LABs present on the PHL-1 culture were isolated from different intestinal sections of healthy poults. Pure cultures of Lactobacillus casei and Lactobacillus bulgaricus were grown on MRS agar and evaluated by agar plate diffusion for bacteriocin-like activity against pathogenic field strains of Salmonella enteritidis PT-13a (SE), Escherichia coli, Campylobacter jejuni, and Campylobacter coli. The two LAB present in PHL-1 culture showed clear inhibition zones against these intestinal pathogens in the agar diffusion technique. Two independent in vivo experiments demonstrated reduction on SE cecal colonization in broiler chickens by prophylactic administration of PHL-1. Studies to clarify the presence of bacteriocins in each isolate as well as in vivo experimeints to identify dose-response of LAB present in the PHL-1 culture are in progress.

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