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United States Department of Agriculture

Agricultural Research Service

Title: Comparison of Four Salmonella Isolation Techniques in Four Different Inoculated Matrices

Authors
item Rybolt, M - MISSISSIPPI STATE UNIV
item Wills, R - MISSISSIPPI STATE UNIV
item Byrd, James
item Doler, T - MISSISSIPPI STATE UNIV
item Bailey, R - MISSISSIPPI STATE UNIV

Research conducted cooperatively with:
item Eka Chemicals

Submitted to: Poultry Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: February 12, 2004
Publication Date: June 1, 2004
Citation: Rybolt, M.E., Wills, R.W., Byrd II, J.A., Doler, T.P., Bailey, R.H. 2004. Comparison of four Salmonella isolation techniques in four different inoculated matrices. Poultry Science. 83:1112-1116.

Interpretive Summary: One of the most important bacteria involved in food poisoning is Salmonella. Poultry and eggs are considered a potential source of human salmonellosis. Because of the need to ensure the safety of poultry products for human food safety, several tests have been developed to detect Salmonella in poultry and poultry houses. Therefore, our laboratory has evaluated four techniques for detecting Salmonella in poultry houses. In this work, we compared three commonly used and one new antibody type Salmonella detection method for poultry and poultry house sources. We compared all four methods in four different sample types. Our conclusions are that the new antibody method was more efficient for detecting Salmonella than the other three commonly used methods. These results will help poultry producers to select an adequate sampling strategy for the detection of foodborne organisms and to eventually reduce the contamination of food products.

Technical Abstract: The introduction of the Pathogen Reduction and HACCP rule has resulted in the poultry industry operating under increased regulatory pressure. These new rules have greatly increased the need for an on-farm food safety risk management program for foodborne bacteria such as Salmonella. Information needed to make informed food safety risk management decisions must be obtained from accurate risk assessments, which rely on the sensitivity of the isolation techniques used to identify Salmonella in the production environment. Therefore, better characterization of the Salmonella isolation and identification techniques is warranted. One new technique, immunomagnetic separation (IMS), may offer a benefit to the poultry industry, as it has previously been shown to be efficacious in the isolation of Salmonella from various sample matrices, including some poultry products. In this work, we compared the isolation ability of four Salmonella-specific protocols: IMS, tetrathionate (TT) broth, Rapport-Vassiliadis R10 (RV) broth, and a secondary enrichment (TR) procedure. All four methods were compared in four different spiked sample matrices: Butterfield's, poultry litter, broiler crops, and carcass rinses. IMS was able to detect Salmonella at a level of 3.66, 2.09, 3.06, and 3.97 log10 CFU/ml in Butterfield's, poultry litter, carcass rinse, and broiler crop matrices, respectively. In the broiler litter and Butterfield's solution, there were no statistically significant (P>0.05) differences among the four isolation protocols. In the carcass rinse and crop samples, there were no differences between the isolation of Salmonella using RV, TR, or TT, but all three were significantly better (P less than or equal to 0.05) than the IMS method.

Last Modified: 4/16/2014
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