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United States Department of Agriculture

Agricultural Research Service

Title: Checmical Characterization of Fusarium Solani F. Sp. Glycines by Image Analysis of Hplc Chromatograms

item Thrane, U - UNIV OF ILLINOIS
item Li, Susan - UNIV OF ILLINOIS
item Hartman, Glen

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: December 10, 2003
Publication Date: August 1, 2004
Citation: Thrane, U., Li, S., Hartman, G.L. 2004. Checmical characterization of fusarium solani f. sp. glycines by image analysis of hplc chromatograms. Phytopathology; 2004.

Technical Abstract: Fusarium solani f. sp. glycines, the cause of soybean sudden death syndrome (SDS), was classified with chromatographic image analysis on full chromatographic matrices obtained by high performance liquid chromatography with UV detection of culture extracts. F. solani non-SDS causing strains were used for comparison. Three chromatograms of each SDS and non-SDS F. solani were selected for calculation of reference chromatograms for identification by chromatographic image analysis. The strains are identified by the cubed cophenetic similarities between each chromatogram and each of the two calculated reference chromatograms, according to the highest similarity. In all cases the chemical identification of the SDS-causing isolates were consistent with the culture morphology and pathogenic identification. Secondary metabolite profiles contained sufficient information for classification and species identification indicating that F. solani f. sp. glycines is a distinct taxon.

Last Modified: 4/22/2015
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