ARS | Publication request: MICROARRAY ANALYSIS OF SUDDEN DEATH SYNDROME IN SOYBEANS

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: October 1, 2003
Publication Date: January 10, 2004
Citation: Li, M., Zou, J., Li, S., Vodkin, L.O., Clough, S.J. 2004. Microarray analysis of sudden death syndrome in soybeans [abstract]. Plant and Animal Genome Conference. p. 273.

Technical Abstract: Soybean Sudden Death Syndrome (SDS) has become a major disease in many soybean-growing states. It can cause 20-80% yield losses under severe conditions. The soilborne fungus, Fusarium solani f.sp. glycines (Fsg) is the causal organism of soybean SDS. The fungus releases toxin that translocates to leaves where it causes distinctive chlorotic and necrotic interveinal patterns. These characteristic foliar symptoms can be reproduced by placing plant cuttings in solution containing Fsg filtrate. The objective of the study was to identify genes with different expression patterns between susceptible and resistant varieties in response to Fsg toxin. Two varieties, Essex (susceptible) and PI567374 (partially resistant), were employed for the study. Plants were grown for 2 weeks under controlled conditions, cut just above the root transition, and placed into Fsg toxin diluted 1:30. Leaf samples were collected at 5 time points: 0, 8, 24, 48, 72 hours post inoculation. cDNA microarray analysis was carried out to check gene expression from a wide array of genes from cDNA libraries derived from various tissues. The results of microarray data analysis at different time points were compared and will be discussed.