COMPARTMENTAL MODELING OF 14C-LYCOPENE IN THE F344 RAT

Authors: Novotny, Janet; ZARIPHEH, SUSAN - UNIV. OF IL; ERDMAN, JOHN - UNIV. OF IL

Submitted to: Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: 4/15/2004
Publication Date: 4/15/2004
Citation: Novotny Dura, J., Zaripheh, S., Erdman, J. 2004. Compartmental modeling of 14c-lycopene in the f344 rat. Experimental Biology.

Interpretive Summary: None

Technical Abstract: Compartmental modeling was used to discern the kinetics of absorption, distribution, and elimination of lycopene in rats. F344 rats were intubated with a single 22 microCi dose of 14C-lycopene (246 microg with 0.5 mL oil) after a 30 day lycopene prefeed of 0.25g lyc/kg diet. Rats (8 per group) were euthanized at 0, 3, 6, 24, 72 and 168 hrs following the dose and tissues were analyzed for 14C. Data from the resulting kinetic curves were incorporated into a multicompartmental model using WinSAAM. Modeling analysis suggested that several tissues receive lycopene directly from chylomicrons, including lung, adipose, adrenal, heart, kidney, testes, and prostate. Further, the kinetics of urinary output suggested that some form of lycopene was eliminated in urine via chylomicrons. Tissues fell into three kinetic groups: faster turnover tissues included heart and spleen; slower turnover, early-peaking tissues included kidney, lung, adipose, liver, and brain; slower turnover tissues with a slightly later peak included testes, prostate, and seminal vesicles. Liver accumulated greater than 10x more lycopene than any other tissue sampled. By understanding the kinetics of absorption, distribution, and elimination of lycopene, one can better hypothesize the physiological functionality of the molecule. ( Funded by USDA/IFAFS Grant # 00-52101-9695)