ARS | Publication request: ANALYSIS OF EXPRESSED SEQUENCE TAGS OF FUSARIUM SOLANI F. SP. GLYCINES DURING INFECTION SOYBEAN /B

Submitted to: APS Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: August 10, 2003
Publication Date: November 1, 2003
Citation: LI, S., HERNANDEZ, A.G., LIU, L., HARTMAN, G.L., DOMIER, L.L., SCHWEITZER, P.A. 2003 Analysis of expressed sequence tags of Fusarium solani f. sp. glycines during infection soybean /B. Am. Path. Soc. Annual Meeting.

Technical Abstract: Fusarium solani f. sp. glycines (Fsg) causes soybean sudden death syndrome(SDS). To identify soybean and Fsg genes preferentially expressed during infection, a normalized directionally-cloned cDNA library was constructed from cultured Fsg and Fsg-infected soybean roots. Fsg and Fsg-infected soybean mRNAs were differentially tagged at the 3'-end for sequence identification. The normalized cDNA library was then subtracted with the cDNA prepared from non-inoculated healthy soybean roots. Initially, 178 cDNA clones were sequenced. Of these, 89 sequences were from cultured Fsg and 78 were from Fsg-infected soybean roots. Ninety-seven (55%) of the expressed sequence tags (EST) significantly matched entries in the National Center for Biotechnology Information non-redundant protein database, 53 were similar to previously identified plant genes, 42 to fungal genes, and two to animal and bacterial genes. Additional EST analysis will provide information on Fsg gene expression during the infection of soybean roots.