|Oliver, R. - PLNT SCI, NDSU, FARGO ND|
|Chen, X. - PLNT SCI, NDSU, FARGO ND|
|Cai, X. - PLNT SCI, NDSU, FARGO ND|
Submitted to: National Fusarium Head Blight Forum Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: November 15, 2003
Publication Date: December 13, 2003
Citation: Oliver, R.E., Chen, X., Xu, S.S., Cai, X. 2003. Identification of novel sources of Fusarium Head Blight resistance from relatives of wheat. National Fusarium Head Blight Forum Proceedings.p. 255. Technical Abstract: Sources of resistance to Fusarium head blight (FHB) are limited. Identification of novel sources is vital to combat this disease. We have screened 284 wheat lines derived from crosses between wheat and its relatives for FHB resistance. Resistance of these materials was evaluated in the greenhouse using the point inoculation method. Approximately 20 spikes were screened for each of the wheat lines. Of the 284 lines evaluated, 100 were highly resistant (<15% infected spikelets), 108 were moderately resistant (15-50% infected spikelets), and 76 were susceptible (>50% infected spikelets). Resistant lines include synthetic common wheat lines, wheat-alien species amphiploids, addition lines, and other wheat-alien species derivatives. To confirm and expand these results, a second season of greenhouse screening is underway. Cytogenetic characterization is in progress to understand the chromosome constitutions of wheat lines with high levels of resistance. Following an initial chromosome count, fluorescence in situ hybridization (FISH) was performed to determine the amount of alien chromatin present in each resistant line. FISH patterns of mitotic chromosomes indicate that four of the most resistant lines carry 14 Thinopyrum ponticum chromosomes and 42 wheat chromosomes. Additional crosses are being made to localize resistance genes, to minimize alien chromatin, and to pyramid FHB resistance genes.