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ARS Home » Southeast Area » Stoneville, Mississippi » Crop Genetics Research » Research » Publications at this Location » Publication #155551
Title: NEAR ISOGENIC LINES CONFIRM A SOYBEAN CYST NEMATODE RESISTANCE GENE FROM PI 88788 ON LINKAGE GROUP J.

Author
item GLOVER, KARL - SO DAKOTA STATE UNIV
item WANG, DECHUN - MICHIGAN STATE UNIV
item Arelli, Prakash
item CARLSON, SHAWN - UNIV OF ILLINOIS
item CIANZIO, SILVIA - IOWA STATE UNIV
item DIERS, BRIAN - UNIV OF ILLINOIS

Submitted to: Crop Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/6/2004
Publication Date: 5/10/2004
Citation: Glover, K.D., Wang, D., Arelli, P.R., Carlson, S.R., Cianzio, S.R., Diers, B.W. 2004. Near isogenic lines confirm a soybean cyst nematode resistance gene from PI 88788 on linkage group J. Crop Science. 44:936-941.

Interpretive Summary: Soybean cyst nematode, a microscopic size worm that attacks soybean roots, is one of the most destructive pests of soybean. Yield losses caused by the pest can be reduced by planting resistant varieties and rotating soybean with other crops. Conventional breeding for nematode resistance is slow and tedious. In this research, a newly identified genetic tag confirmed a major gene for nematode resistance in the soybean variety Bell. This new genetic tag can be used with previously known genetic tags to speed the development of new soybean varieties that are resistant to this important pest. Newer resistant varieties will reduce yield losses caused by the nematode and provide more profits to soybean producers.

Technical Abstract: Soybean cyst nematode (SCN), Heterodera glycines Ichinohe, is one of the most destructive soybean [Glycine max (L.) Merr.] pests worldwide. Genetically controlled resistance in cultivars will reduce yield losses. The most common source of SCN resistance used in northern soybean breeding programs is PI 88788. Genetic tests have previously identified a dominant gene Rhg5 in PI 88788. The objective of our research was to map and confirm additional resistance QTLs (Quantitative Trait Loci) in Bell, a cultivar with resistance from PI 88788. One hundred and four F4- derived lines developed from crossing the cultivars Bell and Colfax (susceptible) were tested for associations between 54 molecular markers and resistance to SCN populations PA3 (HG Type 0-) and PA14 (HG Type 1.3-). Three populations of near isogenic lines (NILs) were developed from F4 plants heterozygous for a region on Linkage Group J where a significant QTL was identified. This QTL was given a confirmed designation of cqSCN-003. The effect of cqSCN-003 was diminished in the NIL populations compared to the F4 population. This was partially due to segregation distortion in the F4 population between the regions containing rhg1 and cqSCN-003. These results demonstrate the importance of verifying QTLs in confirmation populations to accurately estimate their effects.