|Chaparro, Jose - U.S. HORTICULTURAL RESRCH|
Submitted to: Annual International Plant & Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: December 20, 2003
Publication Date: December 31, 2004
Citation: Comstock, J.C., Chaparro, J., Tai, P.Y., Edme, S.J., Mccorkle, K.M., Miller, J.D. 2004. The association of microsatellite markers with resistance to sugarcane yellow leaf virus. Annual International Plant & Animal Genome Conference. Interpretive Summary: Preliminary data are presented on sugarcane yellow leaf virus (SCYLV) and the search for molecular markers associated with to SCYLV resistance. Of the 216 microsatellite primers screened, two primer pairs gave a total of three polymorphic bands associated with SCYLV resistance in a sugarcane population of progeny derived form Green German, a susceptible, Saccharum officinarum crossed with Ind 81-146, a resistant, S. spontaneum. The markers appear to be at a distance from the resistance gene(s) and addition primers are being screened to locate other markers closer to the resistance gene(s).
Technical Abstract: A search for molecular markers associated with resistance to sugarcane yellow leaf virus (SCYLV) was initiated using microsatellite primers developed by a project funded by the International Consortium for Sugarcane Biotechnology. The goal was to develop rapid and precise methods to detect SCYLV resistant clones. A total of 216 microsatellite primers were evaluated using pools of 12 susceptible and 11 resistant progeny from a population of 65 progeny from the cross of Green German (susceptible) by IND 81-146 (resistant). This population had been naturally exposed to SCYLV infection for over 10 years at the USDA-ARS Sugarcane Field Station (an area of high incidence of SCYLV) and the disease status of the individual progeny remained constant for the last three years. Bulk segregant analysis was used to screen the microsatellite primers on both parents and the resistant and susceptible bulks of their progeny. Of the 216 primers, 167 gave at least 4 bands, there were 22 superior primers that averaged 14 amplified products for an average of 8 microsatellite bands per primer. Preliminary, results indicate that there are three microsatellite polymorphic bands associated with SCYLV resistance out of a total of 390 scored. Segregation analysis performed on 47 progeny indicated that two SSR loci are linked but are not linked to the other SSR locus. This suggests that multiple genes may be involved in conferring resistance to SCYLV. The primers are being further evaluated using additional clones within the original population and other clones with a known reaction to SCYLV.