Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: January 10, 2004
Publication Date: January 10, 2004
Citation: CLAWSON, M.L., HEATON, M.P., FOX, J.M., CHITKO MCKOWN, C.G., SMITH, T.P., LAEGREID, W.W. CORRELATION OF SRY AND ZFY ALLELES OF THE Y CHROMOSOME IN U.S. BEEF CATTLE. PLANT AND ANIMAL GENOME CONFERENCE PROCEEDINGS. 2004. ABSTRACT NO. P310. Technical Abstract: In cattle, the ability to correctly identify the Y chromosome is essential for accurate sex genotyping of tissues such as embryos and meat. The bovine sex-determining region of the Y chromosome (SRY) resides on the non-recombining part of the Y chromosome and codes for a transcription factor that is critical for testis differentiation. A polymerase chain reaction primer pair was designed to produce a 1348-bp amplicon for SRY specifically in male cattle. This amplicon was sequenced from 96 bulls representing 16 beef and one dairy breed of cattle. Sequence analysis identified four single nucleotide polymorphisms (SNPs). Alleles from each of these SNPs were perfectly correlated. In addition, the SRY alleles are perfectly correlated with those of a previously identified SNP of the zinc finger Y-linked gene (ZFY) in the same panel of 96 bulls (Heaton et al. 2002 Mammalian Genome 13: 272-281). Only two Y chromosome haplotypes were identified from the combined SRY and ZFY genotype data. One haplotype is prevalent in the U.S. Brahman breed and Brahman influenced composites (a limited representation of the Bos indicus lineage), the other haplotype is prevalent in B. taurus breeds.