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United States Department of Agriculture

Agricultural Research Service

Title: Detection of Salmonella from Post-Chill Broiler Carcasses by Rinse Aliquot and Whole Carcass Enrichment Methods

item Bourassa, Dianna - UGA
item Buhr, Richard
item Cason Jr, John
item Berrang, Mark
item Fletcher, D - UGA

Submitted to: Poultry Science
Publication Type: Abstract Only
Publication Acceptance Date: October 21, 2003
Publication Date: January 26, 2004
Citation: Bourassa, D.V., Buhr, R.J., Cason Jr, J.A., Berrang, M.E., Fletcher, D.L. 2004. Detection of salmonella from post-chill broiler carcasses by rinse aliquot and whole carcass enrichment methods. [abstract] Southern Poultry Science Society and Southern Conference on Avian Diseases. Paper No.19.

Technical Abstract: The objective of this study was to compare the recovery of salmonellae using a rinse aliquot method and a whole carcass enrichment method. Broiler carcasses were collected from a commercial processing plant immediately after a single tank immersion chiller. Carcasses were individually bagged and transported to the pilot processing plant on ice. Each bag was opened, 400 mL buffered peptone added, the carcass shaken for one minute, and 30 mL aliquots were removed for salmonellae recovery for the rinse aliquot method. The carcass and the remaining rinsate were aseptically transferred to a resealable bag and an additional 130 mL of buffered peptone was added making a total of 500 mL for the whole carcass enrichment method. The 30 mL aliquots and whole carcasses plus rinsates were incubated at 37 C for 24 h prior to salmonellae recovery. Of the 20 carcasses, only one was found to be salmonellae positive using the rinse aliquot method (5%) and for the same 20 carcasses 10 were positive using the whole carcass enrichment method (50%). The one positive carcass for the rinse aliquot method was also positive for the whole carcass enrichment method. These results indicate that the whole carcass enrichment method can be used to detect salmonellae from carcasses classified as negative by the rinse aliquot method. Keywords: salmonellae, broilers, whole carcass enrichment, rinse aliquot

Last Modified: 4/22/2015