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United States Department of Agriculture

Agricultural Research Service

Title: Development of An Enzyme-Linked Immunosorbent Assay for Beta-Agonist Zilpaterol.

Authors
item SHELVER, WEILIN
item SMITH, DAVID

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: January 1, 2004
Publication Date: May 10, 2004
Citation: Shelver, W.L., Smith, D.J. 2004. Development of an enzyme-linked immunosorbent assay for beta-agonist zilpaterol. EuroResidue V, May 10-12, 2004, Noordwijkerhout, The Netherlands.

Interpretive Summary: Abstract only. No Interpretive Summary needed.

Technical Abstract: Zilpaterol is a beta-adrenergic agonist approved for use in cattle in South Africa and Mexico but not in the EU or USA. Therefore, we developed an ELISA for zilpaterol. Zilpaterol was reacted with ethyl-4-bromobutyrate followed by reflux with 0.1 M potassium hydroxide; the hapten was reacted with bovine serum albumin or keyhole limpet hemocyanin using EDAC. Anti-zilpaterol antibodies were collected from goats. The average IC50 was 3.94 +/- 0.48 ng/mL (n = 25). The antibody did not cross react with N-alkyl [clenbuterol, bamethane, metaproterenol, salmeterol, salbuterol, (-) isoproterenol, and (+) isoproterenol] or di-aromatic (ractopamine, fenoterol, dobutamine, and isoxsuprine) beta-agonists. The zilpaterol ELISA was tolerant of 5% ethanol or methanol; salt (0 - 1M) did not alter the B0 or IC50s. With pH<6 or >8.8 buffer, the IC50s increased relative to ELISAs run at pH 7.4. In summary, a sensitive, specific zilpaterol ELISA has been developed that can serve as a rapid screening assay.

Last Modified: 9/10/2014
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