|Hursting, Stephen - NIH, BETHESDA, MD|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: November 1, 2003
Publication Date: November 1, 2003
Citation: Takahashi, Y., Hursting, D.S., Wang, T.T. 2003. Soy isoflavone genistein modulates expression of an androgen-respnsive gene SPAK in human prostate cancer cells. Proceedings of the 32nd Annual Meeting of the United States-Japan Natural Resources Food and Agriculture Panel Members, Tsukuba, Japan. 313-315. Technical Abstract: Genistein is one of the major isoflavones in soybean and soy products. Both epidemiological and biological studies supported a role for genistein in prevention of prostate cancer. However, the underlying mechanisms remain unclear. In this study we reported identification of the STE20/SPS1-related kinase SPAK, as a novel gene modulated by genistein in prostate cancer cell line LNCaP using the cDNA microarray approach. We further characterized the effect of genistein on SPAK and found treatment of LNCaP cells with genistein at a concentration as low as 1 M led to a reduction in SPAK mRNA level. We also confirmed that SPAK mRNA level can be induced by the synthetic androgen R1881 in a dose-dependent manner (0.1-10 nM). More importantly, treatment with genistein inhibited the effect of R1881 on SPAK mRNA. The results support a role for genistein in modulation of the androgen responsive gene expression in LNCaP cells and may be a mechanism by which the soy isoflavone genistein exert its protective effects against prostate cancer.