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United States Department of Agriculture

Agricultural Research Service

Title: Genetic Diversity among Arborescent Gossypium Species Revealed by Rapd and Aflp.

Authors
item Feng, Chunda - UNIV. OF ARKANSAS
item Mcd. Stewart, James - UNIV. OF ARKANSAS
item Ulloa, Mauricio
item Garcia, E. - SAGARPA-INIFAP, MEXICO
item Godoy, S. - SAGARPA-INIFAP, MEXICO

Submitted to: National Cotton Council Beltwide Cotton Conference
Publication Type: Proceedings
Publication Acceptance Date: January 1, 2003
Publication Date: January 1, 2003
Citation: FENG, C., MCD. STEWART, J., ULLOA, M., GARCIA, E.A., GODOY, S. 2003. GENETIC DIVERSITY AMONG ARBORESCENT GOSSYPIUM SPECIES REVEALED BY RAPD AND AFLP. NATIONAL COTTON COUNCIL BELTWIDE COTTON CONFERENCE. p. 833.

Technical Abstract: Mexico is one of three centers of diversity of the genus Gossypium. G. aridum, as currently circumscribed, is widely distributed in arid and semiarid regions of Mexico. Morphological variation has been observed among G. aridum plants growing under greenhouse conditions. Nine G. aridum accessions from former collection efforts and 24 accessions from a recent collection effort (including 15 putative G. aridum, 1 G. lobatum, 5 G. laxum , 1 G. schwendimanii and 2 G. gossypioides) were used to evaluate the genetic diversity of 'G. aridum' and the phylogenic relationships among arborescent Gossypium species based on RAPD and AFLP. Twenty-seven random 10-mer primers amplified 210 RAPD bands, and sixteen selective primer combinations generated 766 AFLP bands. Among the 976 bands, 102 were monomorphic, 97 were specific to Gossypium Subsection Selera, 35 were specific to Gossypium Subsection Erioxylum, and 33 were specific to recently collected accession US72. Differing numbers of bands specific to other accessions were also observed. The genetic distance between G. gossypioides and the species within subsection Erioxylum ranged from 0.637-0.841. The genetic distance between two well-established species, G. lobatum and G. schwendimanii, was 0.323. US72 was not only genetically distant from Subsection Selera but was also distant from the four species of Subsection Erioxylum. The genetic distances ranged from 0.420 to 0.538, and the dendrogram based on cluster analysis showed that US72 was positioned in the middle of these four recognized species. This suggested an ancestral position but derivation by homoploid hybrid speciation cannot be ruled out. The genetic distances between Oaxaca accessions and accessions from Colima and Jalisco, as well as those between Colima accessions and accessions from other regions, exceeded that between G. lobatum and G. schwendimanii. The genetic distances among Guerrero accessions were less than 0.323, but these materials were separated into different groups by cluster analysis. In Gossypium Subsection Erioxylum, G. schwendimanii was genetically closer to G. laxum, while G. lobatum was closer to G. aridum. 'G. aridum ' materials were split into several groups, indicating that 'G. aridum,' probably encompasses taxa that, in fact, may be distinct.

Last Modified: 8/27/2014
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