Submitted to: ARS Immunology Workshop
Publication Type: Abstract Only
Publication Acceptance Date: December 3, 2003
Publication Date: December 3, 2003
Citation: CRIPPEN, T.L., HE, H., KOGUT, M.H. THE INHIBITION OF NITRIC OXIDE PRODUCTION IN CHICKEN PHAGOCYTES. ARS IMMUNOLOGY WORKSHOP. 2003. ABSTRACT P. 27. Technical Abstract: The production of reactive nitrogen, nitric oxide (NO), has previously been demonstrated to be a major mechanism by which the innate immune system defends against microbial invasion. The purpose of this study was to characterize components of the signal transduction pathway induced by bacterial stimulation of NO for antimicrobial defense by chicken phagocytes. We quantified the production of nitrite by chicken macrophages (HD11 cells) which had been exposed to specific signal transduction inhibitors prior to stimulation by formalin-fixed Enterococcus gallinarium (EG) or Klebsiella pneumoniae (KP). The induction of many antimicrobial peptide genes is known to be regulated by factors of the NF-kappaB family in a toll-like-receptor dependent or independent manner. NF-kappaB is in turn regulated by IkappaB kinase. We found that NO production induced by both EG and KP was reduced in a dose dependent manner by specific inhibitors of NF-kappaB, IkappaB and JAK 3.