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United States Department of Agriculture

Agricultural Research Service

Title: Stimulation of Midgut Stem Cell Proliferation by Manduca Sexta A-Arylphorin.

Authors
item Blackburn, Michael
item Loeb, Marcia

Submitted to: Entomological Society of America Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: October 29, 2003
Publication Date: December 10, 2003
Citation: Blackburn, M.B., Loeb, M.J. 2003. Stimulation of midgut stem cell proliferation by manduca sexta a-arylphorin.. Entomological Society of America Proceedings. Society of Inverterbrate Biology Mtg. San. Francisco, CA. 5/22-5-26

Technical Abstract: Extracts of the green-colored perivisceral fat body of newly ecdysed Manduca sexta pupae stimulate mitosis in midgut stem cells of Heliothis virescens cultured in vitro. Using a combination of cation- and anion-exchange chromatography, we have isolated a protein from these fat body extracts that accounts for the observed stem cell proliferation. SDS-PAGE analysis of the protein results in a single band of 77 kDa. Sequences of tryptic peptides from this protein are identical to internal sequences of the storage hexamer alpha-arylphorin. The alpha-arylphorin isolated by our procedure represents a small fraction of the total arylphorin present in the fat body extract. However, it alone seems responsible for the stimulation of mitotic activity in H. virescens midgut stem cells.

Last Modified: 9/23/2014