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United States Department of Agriculture

Agricultural Research Service

Title: Genetic Linkage Mapping of An Annual X Perennial Ryegrass P0pulation

Authors
item Warnke, Scott
item Barker, Reed
item Jung, Geunhwa - UNIV. OF WISCONSIN
item Mian, M.A. Rouf - NOBLE FOUNDATION
item Saha, M.C. - NOBLE FOUNDATION
item Brilman, L.A. - SEED RESEARCH OF OREGON
item Dupal, M.P. - AMERSHAM BIOSCIENCES
item Forster, J.W. - AGRICULTURE VICTORIA

Submitted to: Journal of Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 1, 2003
Publication Date: June 30, 2004
Citation: Warnke, S.E., Barker, R.E., Jung, G., Mian, M.A., Saha, M.C., Brilman, L.A., Dupal, M.P., Forster, J.W. 2004. Genetic Linkage Mapping of an Annual x Perennial Ryegrass Population. Theor. Appl. Genet. v:109, pp. 294-304.

Interpretive Summary: Male and female molecular marker linkage maps of an interspecific annual x perennial ryegrass mapping population were developed using several different types of DNA markers. DNA differences that are also present in oat and barley and specific differences from tall fescue and other grasses allowed the chromosomes to be numbered relative to wheat and barley. A total of 442 markers were used to construct the maps. The three-generation population structure allowed both male and female maps to be constructed. The male and female maps differed in map length with the male map being shorter than the female map. Regions of unusual segregation were identified in both maps with chromosomes 1, 3, and 6 of the male map showing the highest percentage of unusual segregation. The Seedling Root Fluorescence (SRF) character was mapped to chromosome 1 in both the male and female maps and the 8-hour flowering character was also localized to this chromosome on the female map. These results indicate that chromosome 1 is currently the best location for identifying markers useful in ryegrass species separation.

Technical Abstract: Male and female molecular marker linkage maps of an interspecific annual x perennial ryegrass mapping population were developed using Amplified fragment length polymorphisms (AFLPs) , restriction fragment length polymorphism (RFLPs), random amplified polymorphic DNA (RAPD) simple sequence repeat (SSR), morphological markers, and isozyme markers. RFLP markers from Oat and Barley and SSR markers from Tall Fescue and other grasses allowed the linkage groups to be numbered, relative to the Triticeae, and the International Lolium Genome Initative reference population P150/112. A total of 235 AFLP markers, 81 RAPD markers, 16 comparative grass RFLPs, 106 SSR markers, 2 isozyme loci and 2 morphological characteristics, 8-hour flowering and Seedling Root Fluorescence (SRF), were used to construct the maps. The three-generation population structure allowed both male and female maps to be constructed. The male and female maps differed in map length with the male map being 537 cM long and the female map 712 cM. Regions of skewed segregation were identified in both maps with chromosomes 1, 3, and 6 of the male map showing the highest percentage of skewed markers. The Seedling Root Fluorescence (SRF) character mapped to chromosome 1 in both the male and female maps and the 8-hour flowering character was also localized to this chromosome on the female map. These results indicate that chromosome 1 is the best location for identifying markers useful in ryegrass species separation.

Last Modified: 7/31/2014
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