Technical Abstract: Factors affecting somatic embryogenesis from root explants of Prunus incisa 'February Pink' were investigated. Using a basic embryogenic media of MS salts and vitamins with 10 uM 2,4,-D, the effects of light, growth regulators, amino acids, carbohydrate source, and root explant media were evaluated. Culturing explants in light or dark conditions both resulted in the formation of embryos. Embryogenesis was inhibited by the addition of BA, TDZ, or GA3 to the media. Amino acids were not effective in promoting embryogenesis, and high levels of amino acids inhibited embryogenesis. While sucrose and glucose were effective in inducing embryogenesis, sorbitol and mannitol completely inhibited embryogenesis. Sucrose and glucose also promoted the formation of secondary embryos. Embryos that formed in media containing 4 or 5% sucrose were abnormally shaped and did not fully develop. Embryos that formed in media with sucrose concentrations of 2 or 3% were much more vigorous. Root explants that were induced on media containing 1.0 uM IBA produced more somatic embryos than explants induced on media without IBA. In all, approximately fifty percent of roots induced on medium containing 1.0 uM IBA produced somatic embryos on media containing 10 uM 2,4-D and 3% sucrose.