Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: October 30, 2003
Publication Date: February 15, 2004
Citation: Rajasekaran, K. 2004. Agrobacterium-mediated genetic transformation of cotton. In: Curtis, I.S., editor. Transgenic Crops of the World - Essential Protocols. The Netherlands: Kluwer Academic Publishers. p. 243-254. Technical Abstract: The commercially available genetically modified cottons with insect-resistance and/or herbicide-tolerance traits are the products of Agrobacterium-mediated transformation. Although this method is applicable to a broad range of species and varieties, the success is limited by low regeneration rates with commercially grown varieties. A detailed, reproducible procedure for Agrobacterium-mediated transformation and regeneration of fertile plants has not been published to date. This book chapter provides a step-by-step procedure on cotton transformation and regeneration. This book is to be used as a practical manual by students and scientists alike for genetic transformation of crop plants.