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ARS Home » Midwest Area » St. Paul, Minnesota » Cereal Disease Lab » Research » Publications at this Location » Publication #153257
Title: DEVELOPMENT OF REAL-TIME PCR ASSAYS FOR THE DETECTION OF STEM AND LEAF RUST PATHOGENS OF WHEAT

Author
item Szabo, Les

Submitted to: Fungal Genetics Newsletter
Publication Type: Abstract Only
Publication Acceptance Date: 1/1/2003
Publication Date: 3/18/2003
Citation: SZABO, L.J. DEVELOPMENT OF REAL-TIME PCR ASSAYS FOR THE DETECTION OF STEM AND LEAF RUST PATHOGENS OF WHEAT. FUNGAL GENETICS NEWSLETTER. 2003. v. 50 p. S469

Interpretive Summary:

Technical Abstract: Historically, stem and leaf rusts have been the one of the most devastating pathogens of wheat, with written accounts of epidemics dating back to ancient Greek and Romans. In North America, major epidemics have occurred from the late 1800's through the 1970's. During the cold war, the United States and the Soviet Union studied Puccinia graminis (wheat stem rust pathogen) as a biological warfare agent. Based on the current concern for biosecurity of agricultural crops we are developing Real-time PCR assays for the rapid detection of P. graminis, P. recondita, P. triticina and P. striiformis using a TaqManTM assay system. Current results on the development of Real-time PCR assays for these wheat pathogens will be presented.