Technical Abstract: We tested whether intestinal permeability, bacterial translocation and inflammation are increased in TPN-fed (TPN) compared to enterally (ENT) fed piglets. After 6 d of treatment, piglets were given an oral gavage of permeability markers and urine collected. At 7 d, tissue was collected and bacterial cultures were obtained. Recovery of markers and myeloperoxidase (MPO) activity were higher in TPN versus ENT pigs, but translocation was not different. In another study, we tested whether intravenous glucagon-like peptide-2 plus TPN (GLP-2) can prevent the TPN-induced (TPN) increase in permeability. Recovery of lactulose, but not PEG 4000, was less in the GLP-2 than in the TPN group. Based upon these studies, we conclude that TPN increased intestinal permeability and mucosal inflammation. Moreover, treatment with GLP-2 partially blocked in the intestinal permeability in TPN-fed pigs.