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United States Department of Agriculture

Agricultural Research Service

Title: Variances of Genetic Distance Measurements among Alfalfa Accessions Compared from Several Molecular Marker Types

Authors
item Kisha, Theodore
item Johnson, Richard
item Skinner, Daniel
item Greene, Stephanie

Submitted to: Crop Science Society Of America
Publication Type: Other
Publication Acceptance Date: January 1, 2002
Publication Date: January 1, 2002
Citation: Kisha, T.J., Johnson, R.C., Skinner, D.Z., Greene, S.L. Variances of genetic distance measurements among alfalfa accessions compared from several molecular marker types. Crop Science Society of America. 2002. Abstract p. 175620.

Technical Abstract: Molecular markers have become an accepted and widely used tool for the measurement of genetic diversity, but the accuracy and precision of the information provided by the many types of molecular markers available are often unknown. The marker used may be selected on the basis of cost efficiency, and may be dependent upon the laboratory of the species being analyzed. To examine the efficiency of various marker types in synthetic populations, ninety-six plants from each of the three alfalfa accessions were analyzed using simple sequence repeats (SSR), Amplified Fragment DNA (RAPD) markers, as well as amplified regions of the hypervariable chloroplast DNA. The accessions were collected from Australia, Turkey, and Spain. All marker types used resulted in similar relative relationships among the three populations. This study discusses the variances of genetic distance measurements associated with each marker type.

Last Modified: 7/23/2014