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United States Department of Agriculture

Agricultural Research Service

Title: Comparison of Molecular Marker Types for Characterization of Alfalfa Accessions

item Kisha, Theodore
item Johnson, Richard
item Skinner, Daniel
item Greene, Stephanie

Submitted to: Crop Science Society Of America
Publication Type: Other
Publication Acceptance Date: January 1, 2001
Publication Date: January 1, 2001
Citation: Kisha, T.J., R.C. Johnson, D.Z. Skinner, and S.L. Greene. Comparison of molecular markers types for characterization of alfalfa accessions. Crop Science Society of America. 2001. Abstract p. 200136

Technical Abstract: Ninety-six plants from each of three alfalfa accessions were analyzed to compare the relative efficacy of molecular marker types for germplasm characterization. The three accessions: 'Aragon', 'Hunter Rivers', and 'Yonca', originated in Turkey, Australia, and Spain, respectively. Markers produced from amplified fragment length polymorphism (AFLP), random amplified DNA (RAPD), and amplified fragments of hypervariable regions of the chloroplast genome were compared. Amplification of hypervariable chloroplast DNA produced a higher frequency of low molecular wight markers within the Spanish accessions Yonca, distinguishing it from the other more similar accessions. Continuing research will determine the utility of these marker types for analyzing genetic diversity through cycles of germplasm conservation.

Last Modified: 4/22/2015