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United States Department of Agriculture

Agricultural Research Service

Title: Human Derived Probiotic Bacteria Species Can Regulate Intestinal Responses to Parasitic Nematodes in Pigs

Authors
item Solano-Aguilar, Gloria
item Dawson, Harry
item Ledbetter, Tonya - JOHNS HOPKINS, MD
item Shea Donohue, P
item Schoene, Norberta
item Call, Jeff - EERC/WYNDMOOR, PA
item Beshah, Ethiopia
item Hare Jr, William
item Urban, Joseph

Submitted to: Veterinary Parasitology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: August 6, 2003
Publication Date: October 1, 2004
Citation: Solano Aguilar, G., Dawson, H.D., Ledbetter, T., Shea Donohue, P.T., Schoene, N.W., Call, J., Beshah, E., Hare Jr, W.R., and Urban Jr, J.F. 2004. Human derived probiotic bacteria species can regulate intestinal responses to parasitic nematodes in pigs. Veterinary Parasitology. 125:47-161.

Technical Abstract: Specific microorganisms that alter the microflora of the host by implantation or colonization and exert beneficial effects on host health are defined as probiotics. Our objective was to demonstrate that commercially available human-derived lactic acid producing probiotic bacteria such as Lactobacillus rhamnosus GG (LGG) (ATCC 53103) and Bifidobacterium lactis (Bb12) could colonize the pig intestine and affect the development of immune and physiological responses to mitogens and secretagogues in vitro, and parasitic nematodes in vivo. LGG was detected in the intestinal mucosa and had a positive effect on weight gain, suggesting improved neonatal growth and health, but no effect on differential blood cell counts or serum liver function enzymes. LGG enhanced mitogen-induced production of pro-inflammatory cytokines in PBMC and the intracellular signaling molecule NF-?B in ex vivo PBMC and bronchoalveolar lavage cells. Bb12 did not alter absorption of glucose in the small intestine, but did attenuate Ascaris suum-induced inhibition of glucose absorption. In contrast, responses of colonic epithelial cells to mast cell-derived secretagogues were reduced in Bb12-treated pigs, but were normal in Trichuris suis-infected pigs. Thus, these probiotics can modulate immune function and selectively affect local responses to parasitic infection while promoting swine health. This model can be extended to assess the activity of selected probiotics on pig responses to other infectious agents and allergens that affect swine and humans.

Last Modified: 11/28/2014