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United States Department of Agriculture

Agricultural Research Service

Title: Techniques to Sample Citrus Canker Bacteria (Xanthomonas Axonopodis Pv. Citri)in Windblown Spray

item Parker, P. E. - USDA-APHIS-PPQ
item Bock, C. H. - USDA-ARS-USHRL
item Gottwald, Timothy

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: May 1, 2003
Publication Date: June 1, 2003
Citation: Parker, P. E., Bock, C. H., Gottwald, T. R. 2003. Techniques to Sample Citrus Canker Bacteria (Xanthomonas Axonopodis pv citri) in Windblown Spray. Phytopathology

Technical Abstract: Four techniques were evaluated to sample wind-blown splash from canker-infected citrus plants. Two volumetric cyclone samplers (the PAS450 and Burkard Cyclone) and two passive samplers (funnels and panels) were used. The PAS450 sampled no bacteria under controlled laboratory or field conditions. The Burkard Cyclone consistently sampled spray, but even when powered off (8 ml-1 and 5ml-1 of spray sampled when powered or not, respectively). Both panels and funnels consistently sampled bacteria laden spray (up to 21500 bacteria ml-1) under field conditions. There was a strong positive correlation between panel and funnel catches for total volume sampled (r=0.9871, P<0.001) and bacteria ml-1 (r=0.9780, P<0.001). Although the number of bacteria ml-1 sampled by the Burkard was correlated with both panel (r=0.8220, P=0.007) and funnel (r=0.7862, P< 0.012) catches, the total volume was not (r=0.1851, ns and r=0.1785, ns, respectively). Panels sampled the greatest volume, and effectively collected bacteria-laden wind-blown splash.

Last Modified: 4/21/2015
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