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United States Department of Agriculture

Agricultural Research Service

Title: Comparative Pathogenicity and Genetic Variation of Beauveria Bassiana Isolates from Asian Longhorned Beetle and Other Cerambyids

Authors
item Bauer, L - US FOREST SERVICE
item Liu, H - MICHIGAN STATE UNIV
item Miller, D - US FOREST SERVICE
item Castrillo, Louela - CORNELL UNIVERSITY
item VANDENBERG, JOHN

Submitted to: Society for Invertebrate Pathology Annual Meeting Proceedings
Publication Type: Proceedings
Publication Acceptance Date: August 15, 2003
Publication Date: August 15, 2003
Citation: BAUER, L.S., LIU, H., MILLER, D.L., CASTRILLO, L.A., VANDENBERG, J.D. COMPARATIVE PATHOGENICITY AND GENETIC VARIATION OF BEAUVERIA BASSIANA ISOLATES FROM ASIAN LONGHORNED BEETLE AND OTHER CERAMBYIDS. PROCEEDINGS OF THE ANNUAL MEETING OF THE SOCIETY FOR INVERTEBRATE PATHOLOGY. 2003. v. 36. p. 48.

Technical Abstract: The Asian longhorned beetle (ALB), Anoplophora glabripennis (Motsch.) (Coleoptera: Cerambycidae) is an invasive wood-boring pest attacking hardwood trees in the United States. We are studying the natural enemy complex of ALB in both the US and its native China. Beauveria bassiana is the most prevalent entomopathogen of ALB, causing mortality during all life stages. Genetic analyses of 13 B. bassiana isolates, collected from infected ALB, cottonwood borer (CWB), Plectrodera scalator F., and the spotted pine sawyer (SPS), Monochamus scutellatus (Say), were done using polymerase chain reaction-based random amplified polymorphic DNA using 12 primers; differences in colony morphology were also observed. Nine of the 13 B. bassiana isolates were distinct with four ALB isolates each from Hebei and Gansu provinces of China and New York City and Chicago in the US; two CWB and two SPS isolates from East Lansing, Michigan. The pathogenicity of one ALB isolate was determined for adults of both CWB and SPS, both native cerambycids, using a standard laboratory bioassay. We found the B. bassiana LC50 and LT50 were significantly lower in SPS than in CWB, due in part to the relatively smaller size of SPS when compared to CWB. Studies on the pathogenicity of this isolate in ALB adults are planned. The suitability of CWB as a surrogate species for the study of ALB management using B. bassiana will be discussed.

Last Modified: 9/10/2014