Submitted to: Mid Atlantic Plant Molecular Biology Society Conference
Publication Type: Abstract Only
Publication Acceptance Date: July 23, 2003
Publication Date: July 23, 2003
Citation: Alkharouf, N., Khan, R., Matthews, B.F. 2003. Analysis of expressed sequence tags from roots of resistatnt soybean infected by the soybean cyst nematode [abstract]. Mid Atlantic Plant Molecular Biology Society Conference . p. 30.
The soybean cyst nematode (SCN), Heterodera glycines, is the most devastating pest of soybean in the United States. The resistance response elicited by SCN in soybean is complex, and genes involved in the response to a large extent are unknown and not well characterized. We constructed cDNA libraries made from mRNA extracted from roots of the resistant soybean cv. Peking at 12 hr, two-to-four days, and six-to-eight days post inoculation with the soybean cyst nematode, population NL1-RHp, similar to race 3. Expressed sequence tag analysis of the libraries provides rapid discovery of genes involved in the response of soybean to the nematode. A total of 3454 cDNA clones were examined from the three libraries, of which 25 cDNAs were derived from nematode RNA. The levels of certain stress-induced genes such as SAM22 and glutathione S transferase (GST8) were elevated in the SCN-infected roots relative to uninoculated roots. Early defense response genes, in particular ascorbate peroxidase and lipoxygenase were abundant in the 12 hr library. By six-to-eight days, the expression of most of those genes was not as abundant, while genes coding for unknown proteins and stress-induced proteins continued to be highly expressed. These ESTs and associated information will be useful to scientists examining gene and protein interactions between nematodes and plants.