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United States Department of Agriculture

Agricultural Research Service

Title: Freeze Tolerance Qtl in the Kanota X Ogle Hexaploid Oat Mapping Population.

Authors
item Wooten, David - NORTH CAROLINA STATE UNIV
item Livingston, David
item Murphy, J. Paul - NORTH CAROLINA STATE UNIV
item Holland, Jim

Submitted to: American Society of Agronomy
Publication Type: Other
Publication Acceptance Date: September 20, 2003
Publication Date: October 15, 2003
Citation: WOOTEN, D.R., LIVINGSTON, D.P., MURPHY, J., HOLLAND, J.B. FREEZE TOLERANCE QTL IN THE KANOTA X OGLE HEXAPLOID OAT MAPPING POPULATION.. GORDON RESEARCH CONFERENCE PROCEEDINGS. 2003.

Interpretive Summary: Detection of freeze tolerance Quantitative Trait Loci (QTL) will provide investigators insight into the inheritance of winter hardiness component traits, and aid the development of winter hardiness marker assisted selection. Kanota is a winter type cultivar while Ogle is a spring type cultivar, resulting in segregation for freeze tolerance in this population and facilitating the detection of freeze tolerance QTL. Extensive research on the Kanota x Ogle mapping population has resulted in the publication of an updated genetic linkage map and generation of population genotype data. We wanted to see if QTL for freezing tolerance could be identified and located within existing linkage groups. In a controlled freeze test of 137 recombinant inbred lines in the F9 generation, crown freeze resistance was normally distributed with a mean of 4.92 and relatively little transgressive segregation. Kanota and Ogle had significantly different freeze tolerances (p<0.05) and two freeze tolerance QTL were detected on Linkage Group 3_23. Three other freeze tolerance QTL were detected in a genome wide scan. The QTL near UMN 220 corresponds with a QTL for vernalization reported previously. This suggests that increased freeze tolerance may result from a pleiotrophic effect of vernalization. The two winter survival genes may be linked as reported in Wheat and Barley. Composite interval mapping proved to be a valuable tool for detecting freeze tolerance QTL linked in repulsion on LG 7_10_28.

Technical Abstract: Detection of freeze tolerance Quantitative Trait Loci (QTL) will provide investigators insight into the inheritance of winter hardiness component traits, and aid the development of winter hardiness marker assisted selection. Kanota is a winter type cultivar while Ogle is a spring type cultivar, resulting in segregation for freeze tolerance in this population and facilitating the detection of freeze tolerance QTL. Extensive research on the Kanota x Ogle mapping population has resulted in the publication of an updated genetic linkage map and generation of population genotype data. We wanted to see if QTL for freezing tolerance could be identified and located within existing linkage groups. In a controlled freeze test of 137 recombinant inbred lines in the F9 generation, crown freeze resistance was normally distributed with a mean of 4.92 and relatively little transgressive segregation. Kanota and Ogle had significantly different freeze tolerances (p<0.05) and two freeze tolerance QTL were detected on Linkage Group 3_23. Three other freeze tolerance QTL were detected in a genome wide scan. The QTL near UMN 220 corresponds with a QTL for vernalization reported previously. This suggests that increased freeze tolerance may result from a pleiotrophic effect of vernalization. The two winter survival genes may be linked as reported in Wheat and Barley. Composite interval mapping proved to be a valuable tool for detecting freeze tolerance QTL linked in repulsion on LG 7_10_28.

Last Modified: 8/27/2014
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