ARS | Publication request: CLONING OF AFLP FRAGMENTS LINKED TO RESISTANCE GENES FOR FUSARIUM HEAD BLIGHT IN BARLEY.

Submitted to: Proceedings International Barley Genetics Symposium
Publication Type: Abstract Only
Publication Acceptance Date: August 30, 2003
Publication Date: June 20, 2004
Citation: Agrama, H.A., Dahleen, L.S., Mcarthur, R.I. 2004. Cloning of AFLP fragments linked to resistance genes for Fusarium Head Blight in barley. Proceedings of the 9th International Barley Genetics Symposium. June 20-26, 2004, Brno, Czech Republic. p. 147.

Technical Abstract: Genetic mapping of resistance genes to Fusarium head blight (FHB) in barley, caused by Fusarium graminearum, revealed multiple locus inheritance. A segregating population of seventy-five double haploid lines, developed from the three-way cross Zhedar 2/ND9712//Foster, was used for genome mapping and FHB evaluation. Four amplified fragment length polymorphism (AFLP) markers were identified and mapped to chromosomes 2H and 5H at regions closely associated with FHB resistance and low deoxynivalenol (DON) concentration. An additional AFLP marker was associated with disease susceptibility. These five AFLP fragments were purified, cloned and sequenced. Sequence-characterized amplified regions (SCARs) will be produced and used for assessing the presence of FHB resistance genes for comparisons in other barley genotypes. These markers may therefore be useful in marker-assisted selection of resistance genes in barley breeding programs and will facilitate map-based cloning.