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United States Department of Agriculture

Agricultural Research Service

Title: Production of Cyclic Alternan Tetrasaccharides from Oligosaccharide Substrates

item Cote, Gregory

Invention: New Molecules from Starch ( Serial Number: 9891123 )

Submitted to: Patent Application
Publication Type: Patent Application
Publication Acceptance Date: June 25, 2001
Publication Date: June 25, 2001
Citation: Cote, G.L. 2003. Production of cyclic alternan tetrasaccharides from oligosaccharide substrates. U.S. Patent 6,562,600.

Technical Abstract: The cyclic tetrasaccharide, cyclo{-6)-alpha-D-Glcp-(1,3)-alpha-D-Glcp-(1,6)-alpha-D-Glcp-(1,3)-alpha-D-Glcp-(1-}, may be produced by alternanase hydrolysis of complex carbohydrates other than alternan. Panose, pullulan, alpha-D-Glcp-(1,6)-alpha-D-Glcp-(1,3)-D-Glc, and D-glucans having alternating alpha-(1,6) and alpha-(1,4) linkages, are all hydrolyzed by alternanase to produce this cyclic tetrasaccharide. In this process, the cyclic tetrasaccharide is produced by contacting a solution of one or more of the above-mentioned complex carbohydrates with an amount of alternanase under conditions effective for activity of the enzyme. The substrate panose used in the reaction may be produced from a variety of polysaccharides or oligosaccharides, including starch, maltose, maltodextrins, pullulan, and mixtures thereof.

Last Modified: 4/22/2015
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