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Title: CLONING AND CHARACTERIZATION OF A TGF-BETA HOMOLOGUE WITHIN POPULATIONS OF ASCARIS SUUM 4TH STAGE LARVAE (L4): REGULATED TRANSCRIPTION AND MULTIPLE SPLICING DIFFERENTIATIVE L4 IN THE JEJUNUM AND ILEUM DURING SPONTANEOUS CURE.

Author
item Zarlenga, Dante
item MORIMOTO, M - BHNRC USDA BELTSVILLE
item Urban, Joseph
item MCCARTER, J - ST.LOUIS MO

Submitted to: World Association for the Advancement of Veterinary Parasitology
Publication Type: Abstract Only
Publication Acceptance Date: 6/15/2003
Publication Date: 8/15/2003
Citation: Zarlenga, D.S., Morimoto, M., Urban Jr, J.F., Mccarter, J.P. 2003. Cloning and characterization of a tgf-beta homologue within populations of ascaris suum 4th stage larvae (l4): regulated transcription and multiple splicing differentiative l4 in the jejunum and ileum during spontaneous cure [abstract]. World Association for the Advancement of Veterinary Parasitology.

Interpretive Summary:

Technical Abstract: Ascaris species represent the most prevalent parasitic worm infecting humans and swine worldwide. During the infection process, Ascaris suum L4 establish in the jejunum and develop into adults. However, a large percentage of L4 in the jejunum spontaneously cure 14 to 21 days after inoculation and are eventually expelled from the intestine. Increased expression of L4 genes for structural proteins related to parasite vigor were detected by cDNA microarray analysis, but gene products that alter host responses to parasitism have not been detected. To this end, a jejunum L4-derived EST library was generated and a putative TGF-ß homologue was identified and characterized by sequence similarity to the Brugia malayi Tgh-1gene and the TGF-ß-like growth factor from Caenorhabditis elegans. Partial sequence information from enzymatically-amplified cDNA revealed at least 2 differentially spliced transcripts. PCR analysis of transcript levels revealed similar quantities of the TGF-ß homologue in all larval stages analyzed except the L4 from the ileum where levels were remarkably lower. Given that mammalian TGF-ß is anti-inflammatory and down regulates the intensity of immune and inflammatory responses, these data support a relationship between L4 avoidance of spontaneous cure and the ability to modify local immunity. Characterization of these genes and their products may provide useful information on the infection process and in designing new control strategies.