ARS | Publication request: IDENTIFICATION AND MAPPING OF AFLP MARKERS LINKED TO FALL ARMYWORM RESISTANCE GENES IN ZOYSIAGRASS

Submitted to: Molecular Breeding of Forage and Turf Conference
Publication Type: Abstract Only
Publication Acceptance Date: April 10, 2003
Publication Date: May 22, 2003
Citation: RENEGANAYAKI, K., ENGELKE, M.C., GENOVESI, A.D., REINERT, J.A., BURSON, B.L., HUSSEY, M.A. IDENTIFICATION AND MAPPING OF AFLP MARKERS LINKED TO FALL ARMYWORM RESISTANCE GENES IN ZOYSIAGRASS. MOLECULAR BREEDING OF FORAGE AND TURF CONFERENCE. 2003. ABSTRACT P. 12.

Technical Abstract: Zoysiagrass, Zoysia matrella (L.) Merr., is a common warm season turfgrass used on golf courses, parks, athletic fields, and home lawns bexause of its drought tolerance, cold hardiness, shade toleracne, salt tolerance and excellent turf quality. However, it is susceptible to number of insects and diseases. One of the insects which may cause economically important damage in this grass as well as others monocots is the fall armyworm, Spodoptera frugiferda (J.E. Smith). Zoysiagrass germplasm with resistance to this insect has been discovered in one important cultivar, 'Cavalier'. Therefore efforts were initiated to breed desirable zoysiagrass turf types that have resistance to fall armyworm. Amplified fragment length polymorphism (AFLP) markers and bulk segregant analysis were used to identify and map the loci controlling resistance. An F1 mapping population from a cross between 'Cavalier' (resistant) and 'Diamond' (susceptible) was developed and used for this study. The individuals in this population segregated for resistance and susceptibility to the insect. DNA samples were bulked from 10 resistant versus 10 susceptible 'Cavalier' x 'Diamond' progeny to make up the respective bulks. These two bulks were subjected to AFLP analysis. A total of 144 fluorescent labeled Pstl/Msel AFLP primer pairs were applied to the bulks. Twenty-eight primer pairs revealed 41 polymorphisms between the bulks. These polymorphisms were tested on the mapping population and the results will be presented.