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United States Department of Agriculture

Agricultural Research Service

Title: Variability of the sugarcane mosaic virus complex in Louisiana

Authors
item Grisham, Michael
item Pan, Yong-Bao

Submitted to: International Society of Sugar Cane Technologists Pathology Workshop
Publication Type: Abstract Only
Publication Acceptance Date: May 20, 2003
Publication Date: N/A

Technical Abstract: Leaf samples from approximately 350 sugarcane plants showing mosaic symptoms were collected in 2002 from 10 locations throughout the Louisiana sugarcane industry. Virus isolates associated with the diseased plants were identified using reverse transcriptase-polymerase chain reaction (RT-PCR) to distinguish between SCMV and SrMV and a restriction fragment length polymorphism (RFLP) analysis of the RT-PCR product to identify strains within each virus (Yang and Mirkov 1997). SrMV strains I and H were associated with approximately 65% and 21% of the plants with mosaic symptoms, respectively. In surveys conducted between 1990 and 1995, over 90% of the plants sampled were infected with SrMV strain H, and the remaining with SrMV strain I except for an occasional sample with SrMV strain M. No SCMV strain was associated with any diseased plant collected during the 1990-1995 or 2003 surveys. RT-PCR showed that approximately 8% of the samples collected in 2002 were infected with SrMV, but the RFLP banding pattern did not match any known strain. The nucleotide sequence of the PCR product and pathogenicity tests of these isolates are being analyzed to determine if a possible new strain of SrMV is responsible for causing these infections. Results of these tests will be presented. No RT-PCR product was produced by either the SCMV or the SrMV primer set for approximately 6% of the plants showing mosaic symptoms suggesting that another virus may cause mosaic in sugarcane in Louisiana. (Yang, Z.N. and Mirkov, T.E. 1997. Phytopathology 87:932-939)

Last Modified: 8/27/2014
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