ARS | Publication request: MOLECULAR CHARACTERIZATION OF THE LANGDON DURUM-TRITICUM DICOCCOIDES CHROMOSOME SUBSTITUTION LINES USING TRAP (TARGET REGION AMPLIFICATION POLYMORPHISM) MARKERS

Submitted to: Wheat Genetics International Symposium Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: June 26, 2003
Publication Date: September 1, 2003
Citation: Xu, S.S., Hu, J., Faris, J.D. 2003. Molecular characterization of the Langdon durum-Triticum dicoccoides chromosome substitution lines using TRAP (target region amplification polymorphism) markers. 10th Wheat Genetics International Symposium Proceedings. September, 1-6, 2003, Paestum, Italy. Abstract not published separately - published in proceedings. Vol. 1:91-94.

Technical Abstract: The Langdon durum-Triticum dicoccoides [LND(DIC)] substitution lines have shown promise in improving grain-protein content and disease resistance in durum wheat. To facilitate the transfer of these desirable traits into durum wheat, we are employing the newly developed TRAP (target region amplification polymorphism) marker technique to develop chromosome-specific molecular markers using a complete set of LND(DIC) substitution lines. The TRAP used one fixed primer of known sequence in combination with a random primer to generate polymorphic markers. To date, 166 TRAP markers have been assigned to individual chromosomes. These markers were amplified with fixed primers homologous to 28 wheat EST sequences mapped onto the A and B genomes of common wheat. The markers were more or less evenly distributed among the fourteen chromosomes, ranging from two (3B) to 24 (5B). Most of the markers were assigned to single chromosomes. However, a small proportion of the markers was present on more than one chromosome, suggesting duplication. The results showed that the TRAP marker technique is efficient in generating chromosome specific markers, with an average of three to six markers per primer set. The markers will be useful in genome characterization and in tagging the genes governing desirable traits in T. dicoccoides.