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United States Department of Agriculture

Agricultural Research Service

Title: A Mate Transporter Gene from Phosphorus (P) Deficient White Lupin: Possible Involvement in Proteoid Root Function

item Uhde-Stone, Claudia - UNIV OF MINNESOTA
item Zinn, Kelly - UNIV OF MINNESOTA
item Liu, Junqi - UNIV OF MINNESOTA
item Miller, Susan - UNIV OF MINNESOTA
item Reinders, Anke - UNIV OF MINNESOTA
item Ward, John - UNIV OF MINNESOTA
item Allan, Deborah - UNIV OF MINNESOTA
item Vance, Carroll

Submitted to: International Symposium on Phosphorus Dynamics in the Soil Plant Continuum
Publication Type: Abstract Only
Publication Acceptance Date: June 18, 2003
Publication Date: N/A

Technical Abstract: When grown under low P, white lupin develops proteoid roots: short, lateral, densely clustered rootlets adapted to improve P acquisition. We previously reported the identification of 35 ESTs (expressed sequence tags) differentially expressed in -P proteoid roots, compared to +P and -P normal roots. Here we present the characterization of a gene with strikingly enhanced expression in proteoid roots subjected to P deficiency, nitrogen deficiency, or aluminum toxicity. The deduced amino acid sequence displays homology to MATE (multi drug and toxin extrusion) family proteins from Arabidopsis. Preliminary results of protein immunoblot analysis indicate that the corresponding protein is most abundant in proteoid root membrane fractions. The MATE promoter has been fused to a GUS reporter gene, and we are currently pursuing the analysis of MATE promoter::GUS fusion activity in transgenic alfalfa and Arabidopsis. For functional analysis, the white lupin MATE gene has been expressed in Saccharomyces cerevisiae and is currently being analyzed.

Last Modified: 4/22/2015
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