ARS | Publication request: TRANSFORMATION OF BARLEY WITH TWO ANTIFUNGAL GENES

Submitted to: Congress on In Vitro Biology
Publication Type: Abstract Only
Publication Acceptance Date: April 10, 2003
Publication Date: May 1, 2003
Citation: DAHLEEN, L.S., MANOHARAN, M. TRANSFORMATION OF BARLEY WITH TWO ANTIFUNGAL GENES. CONGRESS ON IN VITRO BIOLOGY. 2003. V. 39. ABSTRACT p. 2000.

Technical Abstract: Insertion and expression of multiple antifungal genes has the potential of increasing resistance to a variety of fungal diseases. Using particle bombardment and bialaphos selection for the bar gene, multiple barley plants containing both a rice thaumatin-like protein (tlp) gene and a rice chitinase (chi) gene were regenerated from three transformation events. Southern analysis confirmed integration of the transgenes into the barley genome. Northern analysis of T0 plants indicated that event 1 did not contain RNA from either gene. Plants from event 2 expressed both genes at high levels, while plants from event 3 showed small amounts of tlp RNA but no chi RNA. Western analysis of T1 progeny confirmed that event 2 lines expressed both genes and event 3 lines expressed only tlp. Homozygous T2 lines have been identified and are being examined for gene expression levels for both transgenes.