|Bae, Hanhong - UNIV OF MINN|
Submitted to: BARC Poster Day
Publication Type: Abstract Only
Publication Acceptance Date: June 7, 2003
Publication Date: June 7, 2003
Citation: Bae, H., Herman, E.M., Sicher Jr, R.C. 2003. Exogenous trehalose induces carbohydrate accumulation, and alters gene and protein expression patterns in arabidopsis thaliana [abstract]. BARC Poster Day. Technical Abstract: Trehalose is a nonreducing disaccharide (1,1 alpha-D glucopyranosyl alpha-D-glucopyranoside) that occurs in most living organisms, and functions as the principal storage carbohydrate and as an important in osmoprotectant in yeast and certain fungi. Trehalose is present in trace amounts in flowering plants and has been shown to be essential for embryo development. Exposure of higher plants to exogenous trehalose inhibits root growth and affects gene expression. In the current study 30 mM trehalose was applied exogenously to Arabidopsis thaliana grown in liquid culture for two weeks. Whole plantlets were analyzed for changes of metabolites, proteins and gene expression. Starch, soluble sugars and glutamate were increased. ATP levels were reduced and glutamine and Chl (a + b) were unchanged between 0 and 72-h growth on trehalose-containing media. Changes of gene expression were detected using DNA micro arrays and proteomic analyses were performed using two-dimensional gel electrophoresis combined with MALDI-TOF. Using these two high-throughput screening methods, over 50 genes have been identified that potentially respond to exogenous trehalose. Ultra-structural changes to cotyledons and roots were detected by TEM and densely stained particles were observed in the apoplast of trehalose-treated seedlings.