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United States Department of Agriculture

Agricultural Research Service

Title: Interactions Between Bnyvv & Bsbmv and Their Effect on Virus Levels in Sugarbeet.

Authors
item Wisler, Gail - UNIV.FL.,GAINSVILLE
item Lewellen, Robert
item Sears, John
item Liu, Hsing Yeh
item Wasson, Jeffery
item Wintermantel, William

Submitted to: Proceeding of International Working Group on Plant Viruses with Fungal Vectors
Publication Type: Proceedings
Publication Acceptance Date: July 20, 2002
Publication Date: July 22, 2002
Citation: WISLER, G.C., LEWELLEN, R.T., SEARS, J.L., LIU, H., WASSON, J.W., WINTERMANTEL, W.M. INTERACTIONS BETWEEN BNYVV & BSBMV AND THEIR EFFECT ON VIRUS LEVELS IN SUGARBEET. PROCEEDING OF INTERNATIONAL WORKING GROUP ON PLANT VIRUSES WITH FUNGAL VECTORS. 2002. p. 9.

Interpretive Summary: Soils naturally infested with cultures of aviruliferous Polymyxa betae and viruliferous P. betae carrying two sugar beet benyviruses BNYVV and BSBMV, alone and in combination, were compared to non-infested soil for their effects on virus content. Two sugar beet varieties were used: a diploid (Rzrz) that carries resistance to rhizomania caused by BNYVV, and a triploid rhizomania-susceptible variety (rzrzrz). The Rz resistance gene to BNYVV did not confer resistance to BSBMV. Titers of BSBMV were significantly higher in single infections than in mixed infections with BNYVV, in both the rhizomania-resistant and susceptible varieties. In contrast, titers of BNYVV were high (8 to 14 times the healthy mean) in single and in mixed infections in the rhizomania-susceptible variety, but were low (ca. three times the healthy mean) in the rhizomania-resistant variety. Therefore, in the absence of BNYVV, titers of BSBMV are high, regardless of the resistance genotype. However, in the presence of BNYVV, titers of BSBMV are low in both varieties, with absorbance (A405nm) readings similar to those of plants grown in non-infested soils. BNYVV may either out-compete or suppress BSBMV in mixed infections, even in rhizomania-resistant varieties in which titers of BNYVV are extremely low.

Technical Abstract: Soils naturally infested with cultures of aviruliferous Polymyxa betae and viruliferous P. betae carrying two sugar beet benyviruses BNYVV and BSBMV, alone and in combination, were compared to non-infested soil for their effects on virus content. Two sugar beet varieties were used: a diploid (Rzrz) that carries resistance to rhizomania caused by BNYVV, and a triploid rhizomania-susceptible variety (rzrzrz). The Rz resistance gene to BNYVV did not confer resistance to BSBMV. Titers of BSBMV were significantly higher in single infections than in mixed infections with BNYVV, in both the rhizomania-resistant and susceptible varieties. In contrast, titers of BNYVV were high (8 to 14 times the healthy mean) in single and in mixed infections in the rhizomania-susceptible variety, but were low (ca. three times the healthy mean) in the rhizomania-resistant variety. Therefore, in the absence of BNYVV, titers of BSBMV are high, regardless of the resistance genotype. However, in the presence of BNYVV, titers of BSBMV are low in both varieties, with absorbance (A405nm) readings similar to those of plants grown in non-infested soils. BNYVV may either out-compete or suppress BSBMV in mixed infections, even in rhizomania-resistant varieties in which titers of BNYVV are extremely low.

Last Modified: 12/26/2014