|Santoso, Adi - PLNT PATH NDSU, FARGO, ND|
Submitted to: American Phytopathological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: May 1, 2003
Publication Date: June 1, 2003
Citation: EDWARDS, M.C., SANTOSO, A. IDENTIFICATION OF THE NUCLEOTIDE SUBSTITUTIONS REQUIRED FOR BARLEY STRIPE MOSAIC HORDEIVIRUS PATHOGENICITY TO BARLEY POSSESSING THE RSM1 GENE. AMERICAN PHYTOPATHOLOGICAL SOCIETY ANNUAL MEETING. 2003. V. 93(6) ABSTRACT p. S75. Technical Abstract: BSMV strains vary in pathogenicity to hosts such as barley and oat. The barley varieties 'Morex' and 'Modjo-1' are susceptible to strain ND18, but possess the rsm1 gene conferring resistance to strain CV42. Using recombinant viruses generated from ND18 and CV42, we mapped the determinants of BSMV pathogenicity to these hosts. Although pathogenicity determinants mapped to the same region previously identified as critical to oat pathogenicity, the specific amino acid substitutions required are not identical. We previously showed that pathogenicity to oat could be conferred to ND18 by as little as a single nt change in the sequence of RNA alpha, resulting in a single amino acid substitution (P-724 to T-724). We now show that pathogenicity to barley is not affected by the P/T substitution and that other amino acid substitutions in the same region are essential. The amino acid substitutions may result in conformational changes that influence the functionality of the alpha-a gene.