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United States Department of Agriculture

Agricultural Research Service

Title: Rapid Diagnosis of Newcastle Disease Virus Using a Real-Time Reverse Transcriptase Pcr Assay

Authors
item Wise, Mark
item Spackman, Erica
item King, Daniel
item Suarez, David
item Seal, Bruce

Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: January 15, 2003
Publication Date: July 19, 2003
Citation: Wise, M., Spackman, E., King, D.J., Suarez, D.L., Seal, B.S. 2003. Rapid diagnosis of newcastle disease virus using a real-time reverse transcriptase pcr assay. American Association of Avian Pathologists.

Technical Abstract: Newcastle disease virus (NDV), also known as avian paramyxovirus 1 (APMV-1), is responsible for one of the most devastating diseases of poultry. Despite the fact that live and inactivated vaccines are widely used the virus remains a potent threat to commercial flocks. Thus rapid diagnosis of NDV in suspected birds would be highly useful in managing an outbreak situation. To this end we have developed a rapid diagnostic assay based on a real-time reverse transcriptase PCR. The assay targets a conserved region of the matrix gene and utilizes a one-step RT-PCR protocol with fluorogenic hydrolysis (TaqmanTM) type probes. A primer/probe set designed to detect all NDVs has been shown to be specific for a broad range isolates, and will not detect other APMV serotypes. A second primer/probe combination, directed toward the phylogenetic group to which the common lentogenic U.S. vaccine strains belong, allows for the differentiation of these strains from the virulent exotic NDV isolates.

Last Modified: 12/18/2014