Submitted to: American Phytopathology Society
Publication Type: Abstract Only
Publication Acceptance Date: March 25, 2003
Publication Date: N/A
Technical Abstract:
To identify genes involved in biocontrol of Fusarium wilt of tomato by nonpathogenic Fusaria, 415 isolates of oxysporum were screened for pathogenicity and biocontrol ability. Biocontrol ability ranged from low to high. Differences in gene expression were studied with differential display analysis by extracting RNA from 2 pathogenic and 2 biocontrol isolates. cDNA was made from RNA by reverse transcription and was amplified using anchored and random primers. The resulting products were separated by gel electrophoresis and fragments visualized with a flourescent scanner. Twenty-two cDNA bands with potential differential expression between the pathogenic and biocontrol strains were identified, excised and sequenced. Bands with high homology to known sequences included an ATP synthase, an ABC transporter, and a lysine permease. ABC transporters can function in detoxification of toxic compounds and have not been previously reported in Fusarium. Studies continue with additional isolates to determine whether these genes are present in the different phenotyes (but expressed differently), as well as studies on the regulation of the ABC transporter.
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