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United States Department of Agriculture

Agricultural Research Service

Title: Effect of C/n Ratios of Production Media on the Survival of Cryptococcus Nodaensis Oh 182.9 after Freeze-Drying.

item Zhang, S - OHIO STATE UNIV
item Schisler, David
item Boehm, M - OHIO STATE UNIV

Submitted to: American Phytopathological Society
Publication Type: Abstract Only
Publication Acceptance Date: August 13, 2003
Publication Date: June 20, 2003
Citation: Zhang, S., Schisler, D.A., Boehm, J.J. Effect of C/N ratios of production media on the survival of Cryptococcus nodaensis OH 182.9 after freeze-drying [abstract]. Phytopathology (American Phytopathology Society Meeting Abstracts). 93(6):S94.

Technical Abstract: Fusarium head blight, caused by Gibberella zeae, is a devastating disease of wheat worldwide. Cryptococcus nodaensis OH 182.9 has been evaluated as an effective biocontrol agent for this disease. Development of a dried product of OH 182.9 would have potential advantages of ease of handling, favorable economics and acceptance by end users. OH 182.9 was grown for 48 and 72 h in semidefined complete liquid media (SDCL) with C/N ratios of 6.5, 9, 11, 15, and 30. Total biomass production and cell survival for 15 d after freeze-drying were evaluated. Biomass production of OH 182.9 was similar for all cell age by medium C/N combinations. In general, cells harvested at 48 h survived freeze-drying better than those harvested at 72 h. Survival of freeze-dried cells was greatest for cells grown for 48 h in SDCL C/N 30 medium. Cells produced in C/N 6.5 medium generally exhibited the poorest survival. The difference in freeze-dried cell populations between superior and inferior treatments was more than 3 log units. Results of biocontrol efficacy will be reported.

Last Modified: 4/21/2015
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