Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: March 5, 2003
Publication Date: June 1, 2003
Citation: LUSTER, D.G., MCMAHON, M.B., CHOI, J.J., FREDERICK, R.D. PROTEIN PROFILES FROM SOYBEAN LEAVES INFECTED WITH SOYBEAN RUST. PHYTOPATHOLOGY. 93:S55. 2003. Technical Abstract: Soybean rust, caused by the Asian species Phakopsora pachyrhizi, has migrated rapidly from its native range in Asia to Africa and South America in recent years. To identify proteins involved in early interactions between P. pachyrhizi and soybean (Glycine max), we conducted two-dimensional protein gel analysis of leaf apoplastic and cellular proteins expressed at discrete times during the infection process. Preliminary experiments focused on apoplastic proteins from the soybean line Komata, which contains the Rpp1 gene and exhibits a resistant (immune) response to several P. pachyrhizi isolates. Apoplast proteins from leaves of Komata primarily clustered in the range of molecular weights between 10-80 kDa across a broad range of pI's from 3-10. Protein profiles are being analyzed from soybeans sampled 24, 72 and 144 h after inoculation. Differentially expressed proteins will be identified by MALDI-TOF mass spectrometry using EST databases from soybean and P. pachyrhizi.