Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: March 15, 2003
Publication Date: June 1, 2003
Citation: CHOI, J.J., FREDERICK, R.D. PROFILING DEFENSE-RELATED GENE EXPRESSION OF GLYCINE MAX CV. KOMATA INOCULATED WITH SOYBEAN RUST. Phytopathology. 83:S16. 2003. Technical Abstract: Soybeans are the most important legume produced in the United States, used for food, feed, and fuel. A devastating soybean rust pathogen, Phakopsora pachyrhizi, is found worldwide except North America and Europe. Currently, there are no resistant cultivars commercially available in the US. Glycine max cv. Komata is an Asian cultivar that shows resistance to several soybean rust isolates. In order to prepare for the arrival of the soybean rust pathogen, it is crucial to understand the molecular mechanisms involved in resistance. As an initial step, a real-time PCR-based approach and a northern blot analysis were used to assess the expression of known soybean defense-related genes during a susceptible and a resistant interaction using two different soybean rust isolates. The defense-related genes used in this study included acidic and basic chitinases, beta 1,4-glucanase, and phenylalanine ammonia lyase (PAL). In the resistant interaction, differences in the transcription levels of these genes were observed within the first twelve hours after inoculation.