Submitted to: Journal of Animal Science Supplement
Publication Type: Abstract Only
Publication Acceptance Date: June 1, 2003
Publication Date: June 22, 2003
Citation: Nonneman, D.J., Rohrer, G.A. 2003. Positional candidate genes for reproductive traits in a meishan-white composite resource population on pig chromosome 10. Journal of Animal Science 81(Suppl. 1):160. Technical Abstract: Sufficient variation in production traits exists in commercial populations of livestock to exploit allelic variation of superior animals to increase production efficiency and improve the quality of livestock products. Identification of predictive markers by constructing dense comparative maps with human and mouse genomes will allow identification of genomic regions that impact production traits in swine. Several quantitative trait loci (QTL) for important reproductive traits (age of puberty, AP; ovulation rate, OR; nipple number, NN; and plasma FSH, FSH) have been identified on the long arm of porcine chromosome 10, which by bi-directional chromosome painting has been shown to be homologous to human chromosome 10p. Because few anchored markers have been placed on SSC10, we wanted to increase the density of known genes that map to this region of the porcine genome. A total of 20 genes on human chromosome 10p were mapped to pig chromosome 10q and 7 genes from human 10q mapped to pig chromosome 14. Genes from human 10p represent 36 megabases (Mb) that correspond to 53 centimorgans (cM) of pig chromosome 10q with an average marker distance of 2.9 cM (2 Mb of human DNA). Gene order was highly conserved within these markers from centromere to telomere of porcine chromosome 10q, as compared to human chromosome 10p, with 1 large rearrangement along the center of pig 10q. The largest gap in the pig map was 16 cM (104-120 cM on the pig map) corresponding to human 10p14 (8-11 Mb), a region which is very gene-poor in the human. The breakpoint for pig chromosomes 10 and 14 was at the centromere of human chromosome 10. Positional candidate genes were identified for AP (aldo-keto reductase, AKR1C), OR (cAMP regulatory element modulator, CREM), FSH (mannose receptor C1, MRC1) and NN (enhancer of polycomb, EPC1). Nucleotide variation in AKR1C, MRC1 and EPC1 is currently being evaluated in the multi-generation reciprocal backcross resource population as markers for quantitative traits.