ARS | Publication request: EVALUATION OF COTTON TRANSFORMATION VIA POLLEN-TUBE PATHWAY

Submitted to: American Society of Agronomy Meetings
Publication Type: Abstract Only
Publication Acceptance Date: October 21, 2001
Publication Date: October 21, 2001
Citation: Kohel, R.J., Yu, J. 2001. Evaluation of cotton transformation via pollen-tube pathway [abstract]. American Society of Agronomy Meetings. 2001 CDROM.

Technical Abstract: A new technique for transformation was reported in China, but the specifics of its application and results were not documented. The technique called the pollen-tube pathway was evaluated in cotton with different genotypes, time of day, and with two plasmids. Winter greenhouse conditions favored the technique, and there appeared to be an optimum time period. Insertion was independent of the genotypes we tested. Bulk PCR analysis of the progeny indicated the presence of the plasmids, but herbicide applications on the plants did not reveal expression of the insertion. The plants that tested positive for the DNA insertion in 2000 were progeny tested in 2001 for segregation and integration of the DNA. Herbicide resistance expression did not confirm active expression of the insertions.