|Ospina Giraldo, Manuel|
Submitted to: Fungal Genetics Newsletter
Publication Type: Abstract Only
Publication Acceptance Date: July 17, 2003
Publication Date: October 17, 2003
Citation: Ospina-Giraldo, M., Jones, R.W. 2003. Characterization of the glucose-6-phosphate isomerase gene in Phytophthora infestans reveals the presence of multiple alleles. Fungal Genetics and Biology 40:197-206. Technical Abstract: Glucose-6-phosphate isomerase (GPI)plays a key role in both glycolysis and gluconeogenesis. Isoforms of GPI are common, and their isozyme patterns are widely used to characteize isolates of P. infestans. Despite the importance of GPI in P. infestans studies, the gene encoding this enzyme has not yet been characterized. Furthermore, it has been suggested that P. infestans contains multiple copies of the gene but this hypothesis remains to be demonstrated. We have cloned and characterized GPI in various isolates of P. infestans as well as in several species of the genus Phytophthora. The gene contains 1,671 bp and encodes a protein with a predicted molecular weight of 60.8 kD. Multiple different alleles were identified and Southern analysis indicated certain P. infestans isolates carry several copies of the gene. Phylogenetic studies revealed that P. infestans GPI is most closley related to sequences from plant and protozoan orgin.