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United States Department of Agriculture

Agricultural Research Service

Title: Serotype Prevalence and Anti-Microbial Resistance of Salmonella Isolated from Dairy Cattle in the Southwestern United States

Authors
item Edrington, Thomas
item Bischoff, Kenneth
item Looper, Michael
item Callaway, Todd
item Genovese, Kenneth
item Jung, Yong Soo
item Anderson, Robin
item Nisbet, David

Submitted to: American Society of Animal Science
Publication Type: Abstract Only
Publication Acceptance Date: June 23, 2003
Publication Date: N/A

Technical Abstract: Mature dairy cattle were sampled over a two-year period (2001 - 2002) on five farms in New Mexico and Texas. Fecal samples were collected via rectal palpation, cultured for Salmonella, and one isolate from each positive sample serotyped. Three isolates of each serotype, with the exception of Salmonella Newport (n = 10), were examined for antimicrobial susceptibility using the broth micro-dilution technique. Twenty-two different serotypes were identified from a total of 393 Salmonella isolates. Montevideo was the predominant serotype (27%) followed by Mbandaka (15%), Senftenberg (11.4%), Newport (6.4%), Anatum (4.8%) and Give (4.8%). Salmonella Typhimurium and Dublin, two frequently reported serotypes, accounted for only 1% of the observed serotypes in this study. Sixty-four percent of the serotypes were susceptible to all of the antimicrobials, 14% were resistant to a single antibiotic, and 22% were multi-resistant (2 to 11 types of resistance). All isolates tested were susceptible to amikacin, apramycin, imipenem, ceftriaxone, nalidixic acid, and ciprofloxacin. The most frequent types of resistance were to sulfamethoxazole, tetracycline, streptomycin, kanamycin, chloramphenicol, and ampicillin (ranging from 8.9 to 22.4%). Serotypes demonstrating multiple resistance included Dublin and Give (resistant to 3 or more antibiotics), Typhimurium (resistant to 5 antibiotics) and Newport (4 and 2 isolates resistant to 6 and 9 antibiotics, respectively). Class 1 integrons were present in only three isolates, two S. Dublin and one S. Newport. The most prevalent resistance patterns observed in this study were for antimicrobial agents commonly used in cattle, while all Salmonella isolates were susceptible to ceftriaxone and ciprofloxacin, antibiotics used in human medicine.

Last Modified: 9/22/2014
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