ARS | Publication request: GENE EXPRESSION PROFILING OF SOYBEAN IN RESPONSE TO NODULATION

Submitted to: Plant Animal and Microbe Genomes Conference
Publication Type: Proceedings
Publication Acceptance Date: January 15, 2003
Publication Date: N/A

Technical Abstract: Micro array analysis was used to examine genetic responses associated with nodulation in soybean. The roots of 5-day old seedlings were inoculated with Bradyrhizobium japonicum and were harvested at 0, 3, 6, 12, 24, 48, and 72 hrs after inoculation. Harvested roots were immediately frozen in liquid nitrogen and treated for RNA extraction in Trizol reagent. RNA from inoculated and mock-inoculated controls was pooled from approximately five plants for each time point and labeled with either dUTP-Cy3 or dUTP-Cy5 using reverse transcriptase. All samples were also reverse labeled producing at least two reps per time point. Labeled samples were hybridized on cDNA micro arrays representing soybean ESTs from various cDNA libraries, including libraries of B. japonicum inoculated roots. Expression results will be summarized.